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PMC11371747
Interestingly, with the least sensitive cell line, LS174T, potentiation by dl-LV was more pronounced than with l-LV alone.
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PMC11695546
Unique and potent antimicrobial medications for the management of biofilms have been appeared in response to the growing crisis of antibiotic resistance and formation of biofilms of bacteria.
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PMC9429973
In particular, there was a trend towards higher frequency, but reduced intensity of pain crisis in patients with SC disease, but this did not reach significance.
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PMC10135767
Initially, a coating zone (25 mm) covered with clean silicon rubber was designated at the center of each cover slide (Thermo Fisher Scientific, Shanghai, China).
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PMC11802929
We explored this concept by projecting each cell’s antigen specificity (Pep-Groups) onto the GEX-UMAP (Fig. 4d top).
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PMC6600253
However, TNKS1 can induce poly ADP-ribosylation of TRF1, which dissociates TRF1 from telomeric DNA, and results in the exposure of the telomere to telomerase and the promotion of telomere elongation .
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PMC11653552
In vitro studies on human adipose-derived stem cells (ADSC1 and ADSC2) confirmed that osteogenic differentiation is linked to enhanced mineralization and upregulation of osteoblast markers, with increased TAZ expression and decreased Che-1 and miR-590-3p during differentiation.
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PMC11397654
It has already been demonstrated that calixarene derivatives can be biologically or pharmacologically active compounds.
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PMC11775197
PPARβ/δ is widely distributed in various tissues, with the highest expression levels observed in colonic epithelial cells, skin, and adipocytes.
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PMC9429973
Probability of OS was significantly higher in the low-mEASIX group compared to high-mEASIX group (37% vs 25.2%; p=0.008; HR: 2.057; 95% CI: 1.208-3.504).
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PMC9436459
The synthesis featured an enzymatic intermolecular [4 + 2] cycloaddition of different substrates to produce the natural MDAAs and their derivates with a high efficiency and enantioselectivity.
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PMC11680562
Table 3Physicochemical characteristics of the PXFCu gelFormulation codeAppearanceHomogeneitypHSpreadability (cm)PXFCu1Translucent blackGood4.1 ± 0.136.9 ± 0.16PXFCu2Translucent blackGood4.2 ± 0.076.5 ± 0.12PXFCu3Translucent blackGood4.5 ± 0.116.5 ± 0.16PXFCu4Translucent blackVery Good4.2 ± 0.116.4 ± 0.22PXFCu5Translucent blackGood4.5 ± 0.146.4 ± 0.08PXFCu6Translucent blackVery Good4.6 ± 0.186.1 ± 0.16(n = 3), Data represented as mean ± S.D Physicochemical characteristics of the PXFCu gel (n = 3), Data represented as mean ± S.D The drug excipient compatibility investigation used FTIR analysis, a prevalent method for evaluating chemical interactions among various constituents.
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PMC11706776
These results were confirmed by comparing KCNN1, KCNN2, and KCNN3 expression in seven ES and six OS cell lines (GSE229906, Supplementary Fig. 1E, F).
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PMC10919056
OS analysis results in the testing set. (
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PMC9429973
Results: Median age at diagnosis was 68 years (range, 24-88) and 146 pts (60%) were male.
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PMC9429973
At TP3 and TP4 Nabs showed a significant decrease but remained in protective levels for the majority of the patients (mean 88.75% ±9.7 and 74.64% ±17.2 respectively(p<0.0001).
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PMC11468365
After capturing the two tasks’ relationship features, the prediction scores are learned by a prediction subnetwork.
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PMC11740907
In the time-course investigation, samples and a buffer solution containing 1 mm EDTA and 300 mm NaOH at a pH of 13 were placed in horizontally oriented electrophoretic-agarose chambers.
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PMC9268620
Lymphoid precursor cells, namely lymphoblasts, that undergo cell cycle arrest during early cancer development become the precursor malignant cells for lymphoblastic leukemia .
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PMC7215832
Hyper-methylation of tumor-suppressor gene (TSG) promoters is known to have implications in cancer progression and is of therapeutic interest, as hypermethylation of TSG promoters causes suppression of gene transcription and silencing of TSGs whereas CpG demethylation results in TSG transcriptional activation .
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PMC6442998
PDE3A, but not PDE3B, mRNA level was lower in GIST48 compared to GIST882 cells (Figure 2A).
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PMC11252033
Here, we investigated the role of CryZ as a novel therapeutic target in A2780 ovarian carcinoma cells by modulating the protein activity with acetylsalicylic acid (ASA) to restore chemosensitivity.
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PMC9341513
The chromatin concentration is roughly matched to that of a typical nucleus.
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PMC9919300
The most significative differences, shown by comparison between the treated and nontreated groups, reported in Figure 5, evidenced that the chosen treatments were effective in regulate key genes in hepatic lipid content and homeostasis.
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PMC2614416
Oxidoreductase enzymes are extremely diverse in their structures, functions, cellular distribution and biochemical transformations they mediate.
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PMC10778532
We attempted to analyze the consequences of the VHL-C162F mutation in ccRCCs using the clear-cell renal carcinoma 786-0 cells (VHL−/−) transfected with either empty (EV), WT VHL, or VHL-C162F encoding vectors.
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PMC10607604
VCP, a PTPN13 substrate , plays an important role in the regulation of DNA damage repair , although the effect of its dephosphorylation by PTPN13 remains unknown (for review, ).
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PMC11733919
This approach would allow one to document whether CD26 levels are affected in different senescent cell types using different senescence-inducing conditions and to test whether CD26 can be considered as a more common or more private marker of senescence (universal or unique marker of senescence, respectively).
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PMC7709199
For murine IL-12B, the reference sequence (NM_001303244.1) encoding the full-length protein (residues Met1–Ser335) was synthesized by GeneArt and was a kind gift from Complix n.v.,
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PMC11332076
Transient transfection in both cell lines was performed using Polyethylenimine HCl Max (Polyscience).
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PMC11641630
BCG and M. brumae cells were resuspended in sterile phosphate buffered saline (PBS) and adjusted to a 1.0 McFarland turbidity standard as previously described to get the required multiplicity of infection (MOI) for each experiment.
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PMC9429973
Females were additionally censored at date of relapse or stem cell transplantation due to the limited accessibility of assisted reproductive techniques for female patients experiencing one of those events.
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PMC9429973
The drug response data is integrated with patient-matched serum cytokine profiling, RNA-sequencing, and clinical data, allowing to identify the cellular subpopulations whose functional response is predictive of treatment response.
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PMC11757230
Representative bioluminescence images on days 7, 14, and 21 showing tumor growth in CLC/fLuc cell xenotransplantation mouse models treated with different doses of cADSCs. (
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PMC5833712
The role of KDM6B has been extensively studied in development, cell plasticity, immune system, neurodegenerative disease, and cancer.
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PMC10142392
In addition to this variation in crystallinity, degradation by bacterial and fungal enzymes is one of the parameters that has highlighted the interest in PCL, making it particularly attractive for biodegradable material applications.
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PMC11591794
The results from these studies show that GA induces cell death in tumor cells.
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PMC11747467
Interestingly, ERBB2 was mainly enriched in the cell clusters expressing GATA3 and FOXA1 ( Supplementary Figure S3F ; Figure 1D ).
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PMC10968586
Hypoxia increased HT cytotoxicity in MCF-7 cell line, an effect that became evident at 400 μM (vs. 600 μM in normoxic conditions).
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PMC11496491
The hybridized chip was scanned by the scanner and the scan map is obtained.
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PMC11758416
Optional: Rinse the wells with 1 mL of fresh complete media to maximize cell count.
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PMC11803210
Naringenin (Nar) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St Louis, MO).
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PMC11740207
Disruptions in this mechanism can contribute to malignancies, as uncontrolled SD may lead to an accumulation of undifferentiated cells, increasing the risk of tumorigenesis (Chhabra and Booth, 2021; Bolkent, 2024).
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PMC11391695
Furthermore, the encapsulation of nonviral vector-mediated gene delivery systems in nanomaterials coated with tumor cell membranes can prevent unanticipated losses during transport to the target site, enabling effective local gene therapy while minimizing nonspecific diffusion into other tissues .
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PMC11674441
Endotoxin and β-glucan levels were measured using a Toxinometer ET-6000 (Wako Pure Chemical Industries, Ltd., Osaka, Japan), while mycoplasma contamination was assessed with a MycoAlert detection assay (Lonza Rockland Inc., Rockland, ME, USA).
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PMC11657695
Counts were variance stabilized using DESeq2’s rlog transformation function and transformed to z-score.
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PMC11711127
High level of expression of senescence related β-galactosidase has been observed in senescent T cells (85).
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PMC11026382
These structural and biochemical observations are consistent with the finding that mutations with negative epistasis tended to yield moderate to severe growth rate defects.
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PMC10502403
Vδ2CD8 NE1-GMCs secrete IFN-γ in response to PTA.
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PMC9429973
Patients with IL-1RN VNTR non-2-allele carrier genotypes had 1,718-fold increased risk of shorter PFS, but no significant effect on OS was demonstrated.
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PMC11752740
The combination index (CI) values for effective doses were determined based on different fractions of the cell population, following the principles laid out by Chou and Talalay for assessing drug interactions in combination therapies .
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PMC9220170
It is well known that CHOP acts as a transcription factor that, in combination with ATF-4, enhances the expression of various genes.
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PMC11730311
Positive effects on antagonism of hαCGRP8-37 with C20DA-γGlu compared to C18DA-γGlu protraction could be related to a similar mode of action.
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PMC9927933
The difference is statistically significant (P = 0.0241) (Figure 2D), indicating that reduced tumor burden upon Ube2c deletion indeed led to better animal survival.
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PMC11575040
%)OSCHR (95% CI)P-valueAHR (95% CI)P-valueLINC00094(n = 458) Low359 (78.4)1.001.00 High99 (21.6)1.41 (1.04–1.91)0.0271.38 (1.02–1.88)0.036AHR were adjusted for AJCC pathological stage (II, III and IV vs. I)Statistical significance (bold): a two-tailed P-value of <0.05DSS disease-specific survival, DFS disease-free survival, CHR crude hazard ratio, AHR adjusted hazard ratio Univariate and multivariate Cox’s regression analysis of LINC00094 expression for overall survival of patients with melanoma AHR were adjusted for AJCC pathological stage (II, III and IV vs. I) Statistical significance (bold): a two-tailed P-value of <0.05 DSS disease-specific survival, DFS disease-free survival, CHR crude hazard ratio, AHR adjusted hazard ratio In this study, we utilized five melanoma cell lines: A375, A2058, C32, RPMI 7951, and MeWo.
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PMC11699465
We noticed that TMEM199 amplification occurs in multiple cancer types (Figure 1A), including colon cancer, ovarian cancer, sarcoma et al. High TMEM199 expression contributed to worse survival in BLCA, LIHC, and UCEC (Figures 1B–1D).
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PMC9684669
Plates were then washed twice with pre-warmed PBS, filled with 100 µl/well PBS, and measured using a Tecan reader at 485 nm excitation and 535 nm emission.
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PMC11599565
It is worth mentioning that the term “mesoderm” is rather ontogenetic, whereas “mesenchyme” is histologic (embryonic connective tissue).
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PMC11684269
Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a one-carbon (1C) metabolism enzyme, that is involved in modulating de novo purine synthesis.
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PMC11766174
A total of 2 µL of purified RNA was used as template.
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PMC8973725
The 786–0 cells were seeded in 12-well plates (5 × 10 cells/well) and incubated under standard conditions for 1–2 weeks.
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PMC9429973
Methods: We retrospectively reviewed patients diagnosed with DLBCL and treated with R-CHOP from January 2010 to December 2020.
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PMC9429973
The main adverse reactions during the treatment were myelosuppression, of which the incidence of grade 3-4 myelosuppression was 21.43% in newly treated patients and 28.57% in refractory patients.
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PMC9429973
ERCC6L2 affects DNA repair, but also plays a role in immunoglobulin class-switching and mitochondrial function.
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PMC6461034
‒The parameter is calculated by taking a goniometric correlate of the ratio of substrate‐centric and kinase‐centric evidence, and normalizing the corresponding angle on [0,π2] to give a value in .
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PMC11615218
They include cytotoxic T lymphocyte-associated antigen 4 (CTLA4), programmed cell death protein 1 (PD-1), lymphocyte activation gene 3 (LAG3), T-cell immunoglobulin mucin receptor 3 (TIM3), and T-cell immunoreceptor with immunoglobulin and ITIM domains (TIGIT) .
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PMC11429241
While 2D cell cultures offer simplicity, cost-effectiveness, and well-established methods for studying cancer cell behavior, they fall short in accurately replicating the intricate structure of native tumor tissue.
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PMC11525028
Organoids viability was measured using CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, #G7572).
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PMC10882807
Tissues were immediately stored at -80 °C.
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PMC9429973
Results: In this study，88 patients were diagnosed JMML from 2008 till now in our single institute, 44 cases (50%) without treatment (including chemotherapy and HSCT) in the whole course.
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PMC11754432
1ALOX15B is upregulated in Psoriasis.
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PMC11791203
In the current study, the anticancer activity of H. malabarica extracts was evaluated on the human cancer cell line, HT29 and compared with non-cancerous cell line HaCaT. Molecular docking was employed to delineate the possible targets involved in cytotoxicity.
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PMC11731614
Briefly, ion transfer tube temp = 275 °C, Easy-IC internal mass calibration, default charge state = 2 and cycle time = 3 s. Detector type set to Orbitrap, with 60K resolution, with wide quad isolation, mass range = normal, scan range = 375–1500 m/z, max injection time mode = Auto, AGC target = Standard, microscans = 1, S-lens RF level = 60, without source fragmentation, and datatype = Profile.
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PMC11786704
In recent years, there have been significant advancements in polyphenol delivery systems, such as nanoencapsulation and liposome-based delivery systems, which have shown great potential in enhancing the stability and bioavailability of polyphenols .
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PMC9429973
Forty-three (45.3%) patients presented ≥3 HLH-2004 criteria and 34 pts (35.8%) had Hscore>169.
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PMC10197932
500 µL of PI (50 µg/mL, Sigma) and 0,001% NP-40 were added.
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PMC9646302
The potential mechanisms underlying ccRCC were explored in the present study.
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PMC11477621
The flow cytometry data are shown in Figure 4 and Figure 5.
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PMC9587298
For example, the ingestion of natural plants subjected to severe stress, beverages and food products obtained by fermentation and thermally processed foods that might contain considerable amounts of MGO contribute to human exposure to this compound (Oliveira et al., 2021, Yeh et al., 2017).
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PMC11730305
It is the 5th and the 7th common cancer among men and women, respectively.
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PMC10958426
The features of cancer cells and tumor subclones is still not well investigation.
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PMC10170482
A comprehensive landscape of physical parameters and mechanical properties of ECM of the liver is still not fully understood.
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PMC11295144
Quantitative analysis of the % area of synaptic contacts which was normalized to the expression of NL2 for each cell.
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PMC9429973
In clinical trials with myelodysplastic syndromes (MDS)/chronic myelomonocytic leukemia (CMML) patients, DEC-C provides DEC exposures that are equivalent to IV DEC at the approved dose of 20 mg/m daily×5 and is approved as INQOVI® in the US, Canada, and Australia.
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PMC9429973
Methods: A total of 33 patients with chromothripsis detected by CMA were included: 11 with chromothripsis-like patterns (≥7 switches), 22 with classical chromothripsis (≥10 switches).
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PMC11468365
The MolGraphConvFeaturizer extracts a comprehensive set of features that effectively capture the structural and chemical properties of molecules, making it a powerful tool for representing drugs.
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PMC10999934
The estimation of large-scale chromosomal copy number variation (CNV) in somatic cells was performed using the “infercnv” R package .
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PMC11429241
One of the main factors impacting their poor success is the inadequacy of the preclinical 2D culture models for accurately replicating the tumor microenvironment (TME).
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PMC5925824
Table on the right lists shared DEGsacross various tissues with fold-change > 1.7, p value < 0.05 for LY3300054 vs control We describe the discovery and pre-clinical characterization of LY3300054, an Fc effector function-silenced fully human antibody that binds to the PD-L1 checkpoint ligand and blocks interactions with both PD-1 and CD80 receptors.
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PMC11047729
With higher VC concentration, a greater rate of apoptosis was detected .
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PMC9927933
These findings suggested that DEPTOR accumulation, followed by mTORC1/2 inactivation, was causally related at least in part to the inhibition of growth and survival and induction of autophagy triggered by UBE2C knockdown in lung cancer cells.
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PMC11615743
P values were determined by two‐sample equal variance two‐tailed t‐tests ( p < 0.001, p < 0.01, p < 0.05, ns = not significant).
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PMC9429973
The RNA was sequenced on a NovaSeq 6000 (mean 270 million paired-end reads per sample, read length 150 bp).
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PMC11591030
The results were expressed as the mean values ± standard error of the mean (SEM).
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PMC9621239
Second, an explanation is provided for the clinical phenomenon wherein patients infected with genotype C or D HBV have a higher risk of HCC development compared with patients infected with genotype B HBV.
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PMC10890055
However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied.
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PMC11798926
However, how SynDIG4 establishes and maintains these pools is unclear.
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PMC11757230
By contrast, Ahn et al. showed that human ADSCs inhibited the proliferation of the mouse TCL cell line EL4 with or without cell-to-cell contact, and Li et al. reported that human UCMSCs promoted the proliferation of the human BCL cell line Raji in a contact-dependent manner.
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PMC11552389
Significantly more autophagosomes were evident in drug-resistant cells as compared to drug-sensitive cells.
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PMC11285179
RA is an active metabolite of retinol.
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